Genetics of Somatic Mammalian Cells: Lethal Antigens as Genetic Markers for Study of Human Linkage Groups
نویسندگان
چکیده
منابع مشابه
Genetics of somatic mammalian cells: lethal antigens as genetic markers for study of human linkage groups.
The antigen that causes killing of at least 98% of a human cell population treated with a 1% solution of a specific rabbit antiserum in the presence of complement is a sensitive genetic marker. The rapid loss of human chromosomes in human-Chinese hamster cell hybrids makes possible a convenient test of linkage relationships with this marker. Hybrid clones with and without the lethal antigen wer...
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A second surface antigen, B(L), lethal in the presence of specific antibody and complement has been identified on some human cells and shown to behave as a good genetic marker. It is autosomal, unlinked to the human A(L) antigen previously described, and unlinked to 15 other human genes. The A(L) antigen, which is linked to the lactic dehydrogenase A gene, is found on the HeLa, the cultured hum...
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A convenient, reliable method for chromosome delineation of animal cells grown as monolayers on glass has been applied to human, opossum, and Chinese hamster cells. Tissue cultured cells from 5 different, normal organs of 7 different human subjects uniformly displayed the expected chromosome number of 46 and showed no variations in morphology or number other than the expected sex differences an...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1971
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.68.12.3102